hepa 1 6 mouse hepatoma cells Search Results


mouse hepatoma hepa 1–6 cells  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare mouse hepatoma hepa 1–6 cells
Investigation of phosphotyrosine profile upon ablation of Dicer A) Phosphotyrosine profile of control and Dicer knockout mice. Ctrl1 and Ctrl2 represent liver lysates from two control mice and KO 1 and KO 2 represent liver lysates from 2 Dicer knockout mice. The lysates were immunoblotted using anti-phosphotyrosine antibody. B) Schematic representation of workflow followed to identify differentially phosphorylated tyrosine sites upon ablation of Dicer. Pooled liver lysates from 5 control and 5 Dicer knockout mice were spiked with 13C6-lysine and 13C6-ariginine labeled <t>Hepa</t> 1–6 cell lysates and replicate analysis was carried out. Phosphotyrosine containing peptides were enriched using pY100 anti-phosphotyrosine antibody. LC-MS/MS analysis was carried out using LTQ-Orbitrap Velos followed by data analysis.
Mouse Hepatoma Hepa 1–6 Cells, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse hepatoma hepa 1–6 cells - by Bioz Stars, 2026-02
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mouse hepatoma hepa 1–6 cell line  (JCRB Cell Bank)
90
JCRB Cell Bank mouse hepatoma hepa 1–6 cell line
Investigation of phosphotyrosine profile upon ablation of Dicer A) Phosphotyrosine profile of control and Dicer knockout mice. Ctrl1 and Ctrl2 represent liver lysates from two control mice and KO 1 and KO 2 represent liver lysates from 2 Dicer knockout mice. The lysates were immunoblotted using anti-phosphotyrosine antibody. B) Schematic representation of workflow followed to identify differentially phosphorylated tyrosine sites upon ablation of Dicer. Pooled liver lysates from 5 control and 5 Dicer knockout mice were spiked with 13C6-lysine and 13C6-ariginine labeled <t>Hepa</t> 1–6 cell lysates and replicate analysis was carried out. Phosphotyrosine containing peptides were enriched using pY100 anti-phosphotyrosine antibody. LC-MS/MS analysis was carried out using LTQ-Orbitrap Velos followed by data analysis.
Mouse Hepatoma Hepa 1–6 Cell Line, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse hepatoma hepa 1–6 cell line/product/JCRB Cell Bank
Average 90 stars, based on 1 article reviews
mouse hepatoma hepa 1–6 cell line - by Bioz Stars, 2026-02
90/100 stars
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mouse hepatoma cell line hepa1-6-luc  (Shanghai iCELL Biotechnology Co Ltd)
90
Shanghai iCELL Biotechnology Co Ltd mouse hepatoma cell line hepa1-6-luc
Investigation of phosphotyrosine profile upon ablation of Dicer A) Phosphotyrosine profile of control and Dicer knockout mice. Ctrl1 and Ctrl2 represent liver lysates from two control mice and KO 1 and KO 2 represent liver lysates from 2 Dicer knockout mice. The lysates were immunoblotted using anti-phosphotyrosine antibody. B) Schematic representation of workflow followed to identify differentially phosphorylated tyrosine sites upon ablation of Dicer. Pooled liver lysates from 5 control and 5 Dicer knockout mice were spiked with 13C6-lysine and 13C6-ariginine labeled <t>Hepa</t> 1–6 cell lysates and replicate analysis was carried out. Phosphotyrosine containing peptides were enriched using pY100 anti-phosphotyrosine antibody. LC-MS/MS analysis was carried out using LTQ-Orbitrap Velos followed by data analysis.
Mouse Hepatoma Cell Line Hepa1 6 Luc, supplied by Shanghai iCELL Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse hepatoma cell line hepa1-6-luc/product/Shanghai iCELL Biotechnology Co Ltd
Average 90 stars, based on 1 article reviews
mouse hepatoma cell line hepa1-6-luc - by Bioz Stars, 2026-02
90/100 stars
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Investigation of phosphotyrosine profile upon ablation of Dicer A) Phosphotyrosine profile of control and Dicer knockout mice. Ctrl1 and Ctrl2 represent liver lysates from two control mice and KO 1 and KO 2 represent liver lysates from 2 Dicer knockout mice. The lysates were immunoblotted using anti-phosphotyrosine antibody. B) Schematic representation of workflow followed to identify differentially phosphorylated tyrosine sites upon ablation of Dicer. Pooled liver lysates from 5 control and 5 Dicer knockout mice were spiked with 13C6-lysine and 13C6-ariginine labeled Hepa 1–6 cell lysates and replicate analysis was carried out. Phosphotyrosine containing peptides were enriched using pY100 anti-phosphotyrosine antibody. LC-MS/MS analysis was carried out using LTQ-Orbitrap Velos followed by data analysis.

Journal: Biochemical and biophysical research communications

Article Title: Ablation of Dicer leads to widespread perturbation of signaling pathways

doi: 10.1016/j.bbrc.2015.05.077

Figure Lengend Snippet: Investigation of phosphotyrosine profile upon ablation of Dicer A) Phosphotyrosine profile of control and Dicer knockout mice. Ctrl1 and Ctrl2 represent liver lysates from two control mice and KO 1 and KO 2 represent liver lysates from 2 Dicer knockout mice. The lysates were immunoblotted using anti-phosphotyrosine antibody. B) Schematic representation of workflow followed to identify differentially phosphorylated tyrosine sites upon ablation of Dicer. Pooled liver lysates from 5 control and 5 Dicer knockout mice were spiked with 13C6-lysine and 13C6-ariginine labeled Hepa 1–6 cell lysates and replicate analysis was carried out. Phosphotyrosine containing peptides were enriched using pY100 anti-phosphotyrosine antibody. LC-MS/MS analysis was carried out using LTQ-Orbitrap Velos followed by data analysis.

Article Snippet: Mouse hepatoma Hepa 1–6 cells were (kind gift from Kensler Lab, School of Public Health, Johns Hopkins University) adapted to 13 C 6 -Lysine; 13 C 6 -Arginine containing custom DMEM media to label proteins in vivo .

Techniques: Control, Knock-Out, Labeling, Liquid Chromatography with Mass Spectroscopy